Understand the function of a neuron and resolve molecular architecture of neurons in the near-native state to know more about the morphology with in-situ cryo-correlative light and electron microscopy (cryo-CLEM).
Neurodegenerative diseases affect millions of people worldwide. It occurs when neuronal cells in the brain or peripheral nervous system lose function over time, however the molecular architecture of a lot of neurons still remains unclear, such as the structure of α-Synuclein aggregation, a hallmark of Parkinson’s Disease. Cryo-electron microscopy (cryo-ET) is proved to be a powerful tool to investigate how organelles link to each other and how fibrils interact with membranes within neurons at high resolution.
While the traditional cryo-ET workflow is complicated and thus non-efficient, the integrated cryo-CLEM solution by Delmic offers the opportunity to perform in situ cryo-CLEM with the least transfer steps and ice contamination. Researchers are empowered to trace neurons over large distances and identify regions of specific neuronal cell types or automatic detection of areas of interest, with effortless switching between fluorescence light microscope (FLM) and scanning electron microscope (SEM).
The integrated cryo-CLEM solution allows you to obtain high-resolution structural data of neurons in a streamlined cryo-ET workflow. By integrating a fluorescence light microscope(FLM) into your FIB/SEM chamber, you no longer need to transfer your fragile cryo samples between FLM and SEM. Not only are time and effort saved with the integration, but your precious neural samples from animals or even patients are less exposed to the risks of sample damage. Together with our CERES Ice Defence System protecting your samples from ice contamination at every step of the workflow, your sample yield will be dramatically increased.
Anna Bieber and Cristina Capitanio
-Max Planck Institute of Biochemistry
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