Virology is an important section in the field of microbiology. The global impact of viruses is enormous since they cause serious diseases from smallpox to HIV/AIDS and often trigger humanity’s greatest tragedies. By studying viruses, we can understand the way cells work and subsequently learn how to manipulate cells. With the practical findings made in the field of virology, it is possible to gain fundamental insights into the nature of not only viruses but of the cells they
Correlative light and electron microscopy (CLEM) is an effective, direct, and accurate research tool, which can be used to study viruses. Combination of Electron Microscopy (EM) and Fluorescence Microscopy (FM) allows gaining unique insights into the sample in a seamless manner: while studying virus-host interactions, it is possible to move seamlessly from micro to nanoscale level to observe virus replication and membrane modifications at the ultrastructural level.
Fluorescence image of a 100 nm thick ultra-thin section containing whole infected cells acquired using a low magnifica-tion (40X) air objective lens on the SECOM. The sections was mounted on an ITO/glass coverslip and imaged with an excitation wavelength of 467 nm (false colour). Image courtesy of JP Baudoin (IHU–Méditerannée Infection, APHM, Marseille, France).