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CERES Clean Station & Vitri-Lock

Obtain high quality cryo-EM samples and high throughput by minimizing ice contamination

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A common challenge in the cryo-EM workflows is to keep samples vitrified and free from ice contamination. Every scientist knows that the key to high quality data is to have good samples. Crystalline ice formed on or in the sample can obscure the region of interest and significantly lower the cryo-EM data quality.

CERES Clean Station provides a clean and moisture free environment for cryo-EM users to prepare samples with ease. It is designed for high effectiveness with the comfort of the user in mind. 

CERES Vitri-Lock uniquely enables your cryo-sample transfer to take place in a high vacuum, anhydrous environment, to ensure your samples stay uncontaminated during the transfer between the Clean Station and the cryo-FIB/SEM.

Protect your sample against ice contamination

How many times have you ended up with a cryo sample that is contaminated with ice? According to our research, almost 40% of cryo samples become contaminated with ice [1]. The CERES Clean Station and Vitri-Lock keep ice away from your precious sample, allowing you to obtain high resolution structures and make impactful scientific discoveries.

[1] Lau, K., Jonker, C., Liu, J. & Smeets, M. The Undesirable Effects and Impacts of Ice Contamination Experienced in the Cryo-Electron Tomography Workflow and Available Solutions. Micros Today 30, 30–35 (2022).

 

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CERES Clean Station

Prepare cryo-EM samples in the bespoke and highly effective clean station for complete protection against ice contamination.

The CERES Clean Station provides a clean and moisture free platform for cryo-EM users to prepare samples with ease. It is designed for high effectiveness with the comfort of the user in mind. The liquid nitrogen-cooled preparation table consists of three modules specifically designed for:

  1. AutoGrid assembly and shuttle loading
  2. AutoGrid-shuttle transfer
  3. Autoloader cassette loading

Whether you are performing single particle analysis or cryo-ET, the CERES Clean Station is your perfect partner for an ice contamination-free sample preparation.

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Key advantages

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Effective and environmentally friendly

The CERES Clean Station maintains a less than 0.01 % water environment. It consumes significantly less energy than the ineffective and costly low humidity rooms.

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User friendly and ergonomic

The slidable preparation table provides an ergonomic design to perform the different sample preparation steps. The holed sleeves offer users the convenience of working with their preferred gloves.

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Better sample preservation

Use the CERES Vitri-Lock for contamination free sample transfer between the cryo-FIB/SEM and the Clean Station

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Optimize the whole cryo-electron microscopy workflow

Reduce transfer steps and streamline your Cryo-ET sample preparation workflow by combining CERES  Clean Station and Vitri-Lock with METEOR: Delmic's integrated fluorescence microscope.

 

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CERES Vitri-Lock

A high vacuum cryo transfer module that enables safe and ice contamination-free cryo sample transfer

When you have prepared your AutoGrid-shuttle and you are ready to transfer it to your cryo-FIB/SEM for imaging or for lamella preparation, load the shuttle into the CERES Vitri-Lock through the interface without exposing the shuttle to the atmosphere and keep your samples uncontaminated.

After loading the Autoloader cassette with AutoGrids in the Clean Station, place the cassette in the NanoCab to transfer out of the Clean Station through the airlock. CERES Vitri-Lock enables a safe transfer time for up to 30 minutes, ensuring your samples stay vitrified without the need for rushing between stations.

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Key advantages

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Transfer without stress

The CERES Vitri-Lock enables a safe transfer time for up to 30 minutes, ensuring your samples stay vitrified without the need for rushing between stations.

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Obtain better data quality

The CERES Vitri-Lock is pumped to a high vacuum minimizing ice contamination and therefore improving the data quality.

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How does CERES improve your workflow?

How can CERES Ice Defence System be applied to my research?

CERES Ice Defence System is a powerful solution to help you obtain high quality cryo-EM samples and high throughput! It can be applied to a wide range of research areas, such as cell biology and virology. 

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Talk to an expert

CERES Clean Station and Vitri-Lock offers easy and reliable ice contamination-free sample preparation and transfer. Get in touch with our experts to discover how your research can benefit.

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