Cryo-electron microscopy (Cryo-EM) is a technique which received the Nobel Prize in 2017 and revolutionised the process of studying proteins. One of the Cryo-EM methods, called cryo electron tomography, goes one step further. This method includes freezing the cells or tissues at the high speed, transferring them to the EM and then examining at approximately -190°C. After acquiring the series of two-dimensional images, they are then converted into 3D model. While being extremely powerful for studying protein interactions in situ, this method is very laborious and is therefore currently only available to a few laboratories in the world.
Delmic is currently working on a project which is aimed to simplify the complex workflows involved in cryo electron tomography. As a part of a consortium, members of which have years of experience in the fields of cryogenic technologies, vacuum systems and correlative light and electron microscopy, we are working on developing improved solution for the automated sample preparation, contamination-free transfer of samples into the microscope and the archiving of frozen specimens. The new automated workflow CETFlow (Cryogenic Electron Tomography WorkFlow) is aimed for a larger group of users and will unlock cryo electron tomography to a far wider range of users.
Whether you are already doing cryo-ET, or are looking for ways to do it faster and better, contact us directly at firstname.lastname@example.org or by leaving your details below to know more.