Observe fine morphological details while retaining the large context of your sample. Acquire large-scale data and start comparing the sizes and shapes of organelles between cell types or different experimental conditions.
Researchers in cell biology rely on electron microscopy (EM) to answer their research questions since its high resolution is indispensable to visualize the nanoscale details that define individual cells. To analyze various aspects of the complex organization of cells, there is increasing demand to study the same sample at different length scales in biology. Correlative light and electron microscopy (CLEM) is a powerful technique that is used for this purpose. CLEM combines fluorescence microscopy (FM) with high-resolution electron microscopy (EM) to provide information about the composition and function of the sample. However, in traditional CLEM workflows, EM is often a limiting factor for the scope of data collected: with low throughput and limited field of view, careful selection of ROIs is needed to ensure the feasibility and success of a project. Moreover, the limited throughput of the workflow leads to the lack of context, which makes CLEM more of a qualitative technique rather than quantitative.
With high-throughput electron microscopy, CLEM workflows can be enormously simplified and can easily provide quantitative information to the researchers. Delmic’s ultra-fast multibeam scanning electron microscopy, FAST-EM, is capable of collecting megafield of the dimensions of roughly 250 µm x 250 µm, with 4 nm pixel size, within just 3 minutes. This means that you no longer have to compromise between the size and number of samples. Instead of gathering data only on the few labelled regions of interest, it is possible to capture large fields of view with EM and correlate it with the FM data. This type of quantitative analysis typically requires weeks or months to be completed with standard single-beam systems. With FAST-EM it could be completed within a few hours or days.
FAST-EM is highly beneficial for large-scale electron microscopy, where high-resolution imaging is performed on large samples ranging from tissues or organs. Large-scale imaging provides both nanoscale information to analyze subcellular types and the context needed to understand the distribution of cell types within tissues or organs. With FAST-EM you will be able to:
Dr. Jacob Hoogenboom
-Faculty of Applied Sciences, TU Delft
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