Correlative light and electron microscopy (CLEM) is the combination of fluorescence microscopy (FM) with high-resolution electron microscopy (EM). The combination of the
In order to analyze the various aspects of the complex organization of cells, there is an increasing demand to study the same samples at different length scales. Ideally, the researcher would want to obtain a complete overview of a cell and thus require an image on a micrometer length scale, while at the same time analyze biomolecules in that same cell on the scale of a few nanometers. Correlative microscopy enables one to zoom in and out seamlessly on the same sample using an integrated fluorescence and electron microscope.
Figure 1: Image of projection neurons in songbird brain under a correlative light and electron microscope. Imaging was performed using the SECOM platform (DELMIC) mounted on a Quanta 250 FEG SEM (FEI).
The great potential of CLEM lies in the combination of these two modalities: multi-color
With the introduction of the SECOM platform, a completely integrated correlative light
Figure 4: A diagram showing the integrated CLEM system, the SECOM. The electron beam of the scanning electron microscope is shown in green. The added optical light path of the SECOM platform is shown in red.