The SECOM platform is a unique integrated solution for correlative light and electron microscopy that enables you to do correlative microscopy extremely fast with the highest optical quality and overlay accuracy. The combination of the labeling power of fluorescence imaging and the high resolution structural information of electron microscopy makes correlative microscopy the perfect tool to study the complex relation between form and function in biology.
The SECOM is the only product to date that integrates fluorescence and scanning electron microscopy in one device by equipping a scanning electron microscope (SEM) with an inverted fluorescence microscope. The platform can be retrofitted on a SEM by replacing the door to the chamber of the SEM. This replacement supports a motorized stage and the objective and light path for the optical microscope.
Thanks to its integrated design, switching from fluorescence to electron imaging is seamless and instantaneous. And with the automated alignment procedure, you are directly imaging the right location at a high resolution. The SECOM platform comes with an intuitive software package that is designed to easily acquire both types of information. The software package can also control the SECOM stage and the settings for the electron microscope.
The SECOM platform can easily be integrated in the existing workflow and acts as a fully equipped high-end optical microscope, without compromise on either optical or electron performance.
This system is being used worldwide for life science research. See this page for a list of publications for which the SECOM system was used.
DELMIC also produces an integrated CLEM system with a super-resolution optical microscope, the SECOM SR. This system possesses all the features of the SECOM system, but is equipped with an extremely powerful optical microscope for yet more detail down to the nanoscale to study the complex relation between form and function in biology.
The SECOM platform enables accurate and straightforward correlative light and electron microscopy. The integration of two imaging modalities in a single device eliminates the need to switch between the optical and electron microscope and allows for much faster imaging and a more sensible workflow. This enables you to accurately correlate two different types of information on the exact same cell, tissue or structure of interest.
Because only the highest quality optical components are used, the SECOM ensures the best optical performance of any integrated system. Multiband imaging is part of the standard configuration.
At DELMIC we have developed a unique vacuum compatible immersion oil. This allows you to use high-numerical aperture objectives in the SECOM platform. Both water-immersion and oil-immersion objectives are supported.
The SECOM is installed as a retrofit to an existing SEM by replacing the vacuum chamber door. The SECOM is fully compatible with all the standard detectors, allowing you to use the full potential of your electron microscope. Special features such as beam deceleration, in-lens detection and immersion mode are also supported. Like all DELMIC products, the SECOM is compatible with all major SEM manufacturers.