DELPHI, all-in-one solution for correlative microscopy

Load your sample on a transparent conductive coverslip, which is attached to a metal carrier ring using adhesive conductive carbon tape. The sample only needs to be loaded once in the microscope to obtain both fluorescence and electron images. The intuitive software interface means that any user can do correlative microscopy with minimal training. Integration allows immediate focus on analyzing results.

Place the coverslip and the carrier ring on the sample holder. The orientation of the sample is uniquely defined which ensures it is easy to relocate a structure of interest. Then slide the sample holder into place and lower the loading door. This commences the loading procedure which starts with the acquisition of a color navigation image.

DELPHI greatly enhances reliability of the correlative workflow by providing an accurate and unbiased overlay. The key to this alignment procedure is the physical principle of cathodoluminescence. Light is generated where the electron beam hits the glass coverslip and is detected by the camera of the FM and acts as a temporary fiducial marker. A grid of these temporary fiducial markers can be used to produce a perfect overlay, which means that reliable results can be obtained by any user. You can start imaging!

DELPHI is accompanied by a powerful open-source acquisition software ODEMIS, which controls all optical and SEM settings, ensures automated alignment procedure and in just a few clicks lets you visualize overlayed images.