Cryogenic Electron Tomography (Cryo-ET) can provide powerful information to advance the understanding of fundamental life sciences and holds vast promises for biomedical applications. It uniquely resolves tertiary and quaternary biomolecular structures and interactions within the cellular environment. However, the application of cryo-ET to biological research is currently hampered by the requirement of generating very thin lamellae from biological samples, of which the success rate is low due to various technological challenges. Our aim is to overcome these challenges and to provide innovations that dramatically streamline and transform the cryo-ET workflow.