All DELMIC systems come with the specially developed ODEMIS microscopy software. The ODEMIS software extends the capabilities of your microscope by closely integrating hardware with a straightforward software interface. This microscopy software is open-source and makes use of the open file formats OME-TIFF and HDF5. Other powerful tools that improve the imaging workflow include auto-focus, export functionality and a history trail that records previous stage coordinates.
Benefits of ODEMIS software:
For an overview of the version we are currently using and the complete change log, have a look at our Github repository.
Combining different types of data obtained at varying length scales makes correlative microscopy a challenging task. The software package ODEMIS greatly simplifies your correlative imaging workflow as you no longer have to be concerned with overlay accuracy and stage coordinates, leaving time to focus on imaging.
ODEMIS gives you control over both the fluorescence and electron microscope and navigating the sample is easy with either the fluorescence or SEM channel. The user interface is organized into different streams: one for acquiring fluorescence data, one for acquiring SEM data and two combined streams. The fluorescence stream is equipped with a fluorophore database and you can use multiple color channels with individual settings per channel.
To simplify correlative microscopy, ODEMIS features a unique fully automated alignment procedure resulting in accurate, unbiased overlays on every specimen. This overlay is directly visualized in the acquisition software, allowing you to quickly adapt your imaging conditions. (For more information about the automated overlay procedure, you can download our Automated Overlay Technical Note).
ODEMIS allows for the easy acquisition and analysis of data. The software controls the scanning of the electron beam acquiring both secondary electron images and the triggering for the acquisition of spectral and angular resolved images. Spectral response data can immediately be subtracted from the datasets, showing the ‘clean’ spectrum at a glance.
In particular, this microscopy imaging software has several features for the successful and efficient acquisition of cathodoluminescence images. Firstly, it provides drift correction. Secondly, ODEMIS allows for the simultaneous acquisition of the secondary electron and spectral or angle-resolved images. Thirdly, the user can easily obtain images over an arbitrarily sized grid with an arbitrary number of pixels. Finally, the software can easily obtain a large number of angle-resolved images.
ODEMIS is also used in the process of analyzing data. The user can therefore easily visualize 3D CL data as a 2D MAP or pixel-by-pixel graph. The software also provides an advanced 2D slicer for 3D datasets with adjustable integration limits. For angle-resolved images, the software allows for immediate polar plotting. For spectral images, the software overlays the SEM and spectral images. ODEMIS also provides correction files (such as the system response function) to obtain a corrected spectrum in one go. Lastly, the software provides an automated peak fitting feature and files can be easily transferred to, for example, MATLAB for detailed analysis.