DELMIC's in-house application specialists are experts in correlative and cathodoluminescence microscopes, and are continuously working together with scientists in various fields to develop leading innovations in microscopy.

Find here a collection of application notes, technical notes, white papers, and dissertations written by DELMIC's specialists.


Journal articles

de Boer, Pascal, Jacob P. Hoogenboom, and Ben NG Giepmans. "Correlated light and electron microscopy: ultrastructure lights up!" Nature methods 12.6 (2015): 503-513.

Brama, Elisabeth, et al. "Standard fluorescent proteins as dual-modality probes for correlative experiments in an integrated light and electron microscope." Journal of Chemical Biology 8.4 (2015): 179-188.

Debroye, Elke, et al. "Assessing Photocatalytic Activity at the Nanoscale Using Integrated Optical and Electron Microscopy". Particle & Particle Systems Characterization. 33.7 (2016): 412 - 418.  

Huan, Y. et al"Photoluminescence Blinking of Single-Crystal Methylammonium Lead Iodide Perovskite Nanorods Induced by Surface Traps", ACS Omega 1 (1), 148–159 (2016)

Liv, Nalan, et al. "Electron microscopy of living cells during in-situ fluorescence microscopy", ACS Nano 10, 265-273 (2016)

Liv, Nalan, et al. "Simultaneous correlative scanning electron and high-NA fluorescence microscopy." PLoS One 8.2 (2013): e55707.

Liv, Nalan, et al. "Scanning electron microscopy of individual nanoparticle bio-markers in liquid." Ultramicroscopy 143 (2014): 93-99.

Narváez, Angela C., et al. "Cathodoluminescence Microscopy of nanostructures on glass substrates." Optics Express 21.24 (2013): 29968-29978.

Peddie, Christopher J., et al. "Integrated light and scanning electron microscopy of GFP-expressing cells." Methods in cell biology 124 (2014): 363-389.

Peddie, Christopher J., et al. "Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells." Ultramicroscopy 143 (2014): 3-14.

Sueters-di Meo, J., et al. "Using advanced correlative microscopy to study complex biological samples in Encyclopedia of Analytical Chemistry", eds R.A. Meyers, John Wiley: Chichester, a9473 (2016)

Voorneveld, Philip W., et al. "Loss of SMAD4 alters BMP signaling to promote colorectal cancer cell metastasis via activation of Rho and ROCK." Gastroenterology 147.1 (2014): 196-208.

Voortman, Lenard M. "Integration Without Compromise." Microscopy Today 22.06 (2014): 30-35.

Yuan, Haifeng, et al. "Degradation of Methylammonium Lead Iodide Perovskite Structures through Light and Electron Beam Driven Ion Migration." The Journal of Physical Chemistry Letters 7 (2016): 561-566.

Zonnevylle, A. C., et al. "Integration of a high-NA light microscope in a scanning electron microscope." Journal of microscopy 252.1 (2013): 58-70.

Super-resolution correlative microscopy

Chang, Yi-Wei, et al. "Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography." Nature methods 11.7 (2014): 737-739.

Johnson, Errin, et al. "Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins." Scientific reports 5 (2015).

Kopek, Benjamin G., et al. "Correlative photoactivated localization and scanning electron microscopy." PLoS One 8.10 (2013): e77209.

Ligeon, Laure-Anne, et al. "Structured illumination microscopy and correlative microscopy to study autophagy." Methods 75 (2015): 61-68.

Liss, Viktoria, et al. "Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy." Scientific reports 5 (2015).

Löschberger, Anna, et al. "Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution." J Cell Sci 127.20 (2014): 4351-4355.

Paez-Segala, Maria G., et al. "Fixation-resistant photoactivatable fluorescent proteins for CLEM." Nature methods 12.3 (2015): 215-218.

Perkovic, Mario, et al. "Correlative light-and electron microscopy with chemical tags." Journal of structural biology 186.2 (2014): 205-213.

Sochacki, Kem A., et al. "Correlative super-resolution fluorescence and metal-replica transmission electron microscopy." Nature methods 11.3 (2014): 305-308.

Watanabe, Shigeki, et al. "Protein localization in electron micrographs using fluorescence nanoscopy." Nature methods 8.1 (2011): 80-84.

Luminescence database for geological materials
Database of refractive indices
Widgets for calculating transition radiation and surface plasmon generation in a material
Electron interaction Monte Carlo simulator

Inorganic materials

Cathodoluminescence Microscopy of Inorganic Solids, B. G. Yacobi and D. B. Holt, Plenum Press (1990).


Cathodoluminescence in Geosciences, M. Pagel et al., Springer (2000).

Application of Cathodoluminescence Imaging to the Study of Sedimentary Rocks, S. Boggs jr. and David Krinsley, Cambridge University Press (2006).

Zircon, J. M. Hanchar and P.W.O. Hoskin, Rev. Mineral. and Geochem. 53 (2003) 1-500.


Cathodoluminescence microscopy: Optical imaging and spectroscopy with deep-subwavelength resolution, T. Coenen, B. J. M. Brenny, E. J. R. Vesseur, and A. Polman, MRS Bulletin 40, 359 (2015)

Angle-resolved cathodoluminescence nanoscopy, T. Coenen, University of Amsterdam (2014)

Electron beam imaging and spectroscopy of plasmonic nanoantenna resonances, E. J. R. Vesseur, Utrecht University (2011)

Cathodoluminescence plasmon microscopy [PDF], M. Kuttge, Utrecht University (2009)

Electron beams set nanostructures aglow [PDF], Nature 493, 143 (2013)

Electron Energy Loss Spectroscopy imaging of surface plasmons at the nanometer scale, C. Colliex, O. Stephan, and M. Kociak, Ultramicroscopy, DOI:10.1016/j.ultramic.2015.11.012 (2015).

Optical excitations in electron microscopy, F. J. García de Abajo, Rev. Mod. Phys. 82, 209 (2010).

Principles of nano-optics, L. Novotny, B. Hecht, Cambridge University Press (2012)

Classical electrodynamics, J. D. Jackson, John Wiley and Sons (1999).

Our priority at DELMIC is the optimization of research and the dissemination of knowledge. We therefore invite you to contact us in case you have any requests or questions about the above resources. Questions can be directed to Noor van der Veeken at

We produce high-performance microscopy systems for the life sciences and materials science. The SECOM is a correlative light and electron microscope, and the SPARC is a SEM cathodoluminescence system.