Resource library

Software

Open-source software ODEMIS

Download the version 3.2.3, 55.2 MB

White papers

A Molecular View on Neurodegenerative Diseases

Discover how cryo-EM has revealed the underlying molecular pathology of diseases such as Parkinson’s and Alzheimer's, and how this could be the key to unlocking treatment options. 

Customer stories

How New York Structural Biology Center increased their lamellae production 3x without compromising the workflow

Armed with the CERES Ice Shield, the New York Structural Biology Center was able to protect lamellae from ice contamination and achieve both an increase in lamellae production and resolution.

White papers

The Cathodoluminescence Guide: Getting Started with CL

Download our white paper to learn about the five factors influencing CL measurements, so you can get started with imaging right away by using the provided example parameters.

Application notes

Accelerate and deepen the insight into infectious disease with FAST-EM

In the study of life threatening infectious diseases, speed is essential. Delmic's FAST-EM is a high resolution imaging system that provides a 4 nm resolution and up to 100 fold accelerated image collection, meeting the resolution and speed requirements for gaining insights into infectious diseases rapidly.

Brochures

FAST-EM Workflow

Download this material to learn more about each step of the FAST-EM workflow, its advantages and how it can take your research from sample to insights significantly faster. 

Application notes

Minimizing Ice Contamination During Lamellae Fabrication using CERES Ice Shield

Curious to learn how you can create more high-quality lamellae per session and thereby, obtain higher resolution structures? Download the application note where we assess the effect on ice contamination after installation of CERES Ice Shield in three different cryo-FIB/SEMs.

White papers

Correlative cryo-FIB milling strategies using METEOR

Read the white paper where we discuss three different correlative cryo-FIB milling strategies using METEOR. We present test cases and provide guidelines for when to use which strategy.  

White papers

Sample preparation for cathodoluminescence microscopy

Download the white paper and learn about several sample preparation methods that are suitable for cathodoluminescence imaging and compatible with our cathodoluminescence solutions.

Application notes

Type 1 diabetes: Imaging Insights

Learn about integrated correlative light and electron microscopy for studying Type 1 diabetes.

Application notes

Cellular Biology: In-resin Fluorescence Using GFP and mCherry

Read how best overlay accuracy in correlative microscopy can be achieved when acquiring fluorescence and electron images on the same resin-embedded section.

Application notes

Integrated CLEM for Giant Viruses

Read how integrated correlative light and electron microscopy (CLEM) reveals the giant virus particles by fluorescence light microscopy (LM) while simultaneously providing information on the ultrastructure with electron microscopy (EM).

Application notes

Integrated Super-resolution CLEM with Conventional Fluorescent Proteins

Learn how Super-resolution (SR) light microscopy and electron microscopy (EM) together revolutionize our understanding of biological systems, providing highly accurate correlation of fluorescent proteins with cellular structure.

Application notes

Marine Microbiology: an Image-based Approach

Learn about the possibilities of integrated correlative light and electron microscopy for studying and understanding marine microbes.

Application notes

Neuroscience: Synaptic Connectivity in the Songbird Brain

Learn how integrated correlative light and electron microscopy can be beneficial for understanding synaptic connectivity and large-scale interactions in the brain.

Webinars

Real-time in situ correlative microscopy for cellular biology

Learn about the concept of correlative light-electron microscopy (CLEM) and how it can be used for various applications in the life sciences.

Webinars

Integrated CLEM

Learn about the relevance of CLEM in the life sciences, as well as some of the challenges such as overlaying images and retrieving regions of interest. 

Webinars

Cathodoluminescence Fundamentals: Imaging conditions for CL

Learn about how to set up imaging conditions to obtain high quality CL intensity maps and spectra from a range of materials

Webinars

Cathodoluminescence Fundamentals: CL processes

Learn about the difference between coherent and incoherent CL emission, how it can be identified and what each type of the emission can be used for.

Webinars

Giant Viruses under correlative light-electron microscopy

Learn about the possibilities of integrated CLEM for studying giant viruses, which are found in environmental, animal, as well as human samples.

Webinars

Cathodoluminescence for photovoltaics

Learn in details about the possibilities of CL imaging for measuring and analysing thin-film solar cells and various materials used in the photovoltaic industry, this is the webinar for you.

Webinars

Cathodoluminescence spectroscopy for nanoscale optics, geology, and materials science

Learn about how the measurement of cathodoluminescence emission can be applied in nanoscale optics, geology, and materials science.

Webinars

Analytical SEM Solutions for geology

Learn about exploration of microstructure and chemistry in minerals using EBSD and CL techniques.

Webinars

Angle-resolved cathodoluminescence spectroscopy

Learn about the different types of cathodoluminescence (CL) and more specifically, how angle-resolved CL is performed.

Webinars

Cathodoluminescence imaging for geology

Learn more the physical background of cathodoluminescence emission in rocks, sample preparation, and the typical CL workflow.

Webinars

Time-resolved cathodoluminescence

Learn more the properties of ligh, the dynamics in materials, time-resolved imaging techniques (including lifetime imaging and g(2) imaging) and examples of how these technique can be applied to study nanostructured semiconductors.

Webinars

Cathodoluminescence for rare-earth doped materials

Learn more the fundamentals of cathodoluminescence for rare-earth doped materials

Webinars

Cathodoluminescence for plasmonics

Learn how cathodoluminescence can be used to retrieve optical properties of plasmonic materials with the deep-subwavelength resolution.

Webinars

Introduction to cathodoluminescence imaging

A comprehensive overview of the cathodoluminescence technique, latest developments and various applications.

Webinars

Superresolution correlative microscopy

Learn about the principle of operation, details of CLEM, hardware, open source software-based operation and scripting, as well as sample preparation.

Application notes

Cathodoluminescence for Zircons

Read this application note to learn how cathodoluminescence is used as a prescreening tool for high-resolution secondary-ion- mass spectrometry (SIMS) to image the zonation pattern and identify regions of interest for isotope analysis of geological samples, such as zircons.

Webinars

Cathodoluminescence imaging for nanophotonics

Learn more the physical background of CL emission, sample preparation, and the typical CL workflow.

Webinars

What is spectrally and angular-resolved cathodoluminescence?

Learn about the possibilities of energy-momentum cathodoluminescence for acquiring hybrid multidimensional high-resolution angle-resolved hyperspectral datasets in the fields of nanophotonics and materials science.

Application notes

Integrated Photonics: Photonics Crystals

Read how cathodoluminescence can be used for retrieving optical properties of photonic crystals with deep-subwavelength resolution.

Application notes

Imaging Optical Modes in Semiconductor Nanoparticles

Learn why and how cathodoluminescence imaging can be used to directly visualize the internal modal structure of semiconductor nanoparticles.

Application notes

Cathodoluminescence Intensity Mapping

Learn about the fastest and simplest method to obtain cathodoluminescence contrast: intensity mapping.

Application notes

Cathodoluminescence for Bulk and Nanostructured Gallium Nitride-based LED Materials

Learn how cathodoluminescence imaging and spectroscopy is used extensively to study GaN materials and devices.

Application notes

Cathodoluminescence imaging on perovskites: phase segregation in inorganic CsPbIBr₂

Learn how cathodoluminescence imaging and spectroscopy is used to study perovskite materials.

Application notes

Cathodoluminescence for Rare-earth Doped Materials

Rare earth materials are a particular class of elements in the periodic table which serve a range of practical uses. Learn how cathodoluminescence can be used to study these materials.

Application notes

Cathodoluminescence for Thin-film Solar Cells

Learn how cathodoluminescence imaging and spectroscopy is used extensively to study properties of thin-film solar cells.

Application notes

Cathodoluminescence Imaging of Three-Dimensional Methylammonium Lead-Halide Perovskite Structures

Learn how cathodoluminescence (CL) imaging and spectroscopy provides a powerful method to study the optical properties of 3D perovskites structures on deep-subwavelength scales.

Technical notes

SECOM: Automated Overlay

Read this technical note to learn how accurate and unbiased overlay is achieved in SECOM, an integrated correlative light and electron microscope.

Technical notes

Angle-Resolved Cathodoluminescence Imaging

Learn the possibilities of angle-resolved imaging mode available in Delmic CL solutions. 

Technical notes

Hyperspectral Cathodoluminescence Imaging

Learn the possibilities of hyperspectral imaging mode available in Delmic CL solutions.

Technical notes

Polarization-Filtered Cathodoluminescence Imaging

Learn the possibilities of polarization-filtered cathodoluminescence imaging mode available in Delmic CL solutions.

Technical notes

Cathodoluminescence Intensity Mapping

Learn the possibilities of cathodoluminescence intensity imaging mode available in Delmic CL solutions.

Technical notes

Optical Modules in the SPARC Cathodoluminescence System

Read more about the optical modules of the SPARC Spectral and Compact systems, which can be configured for various detection functionalities and wavelength ranges.

Technical notes

Lifetime Cathodoluminescence Mapping

Learn about lifetime (or decay trace) mapping which can help to identify a material defect and to characterize materials to improve their performance.

Technical notes

Large-area Hyperspectral Cathodoluminescence Imaging

Learn about the possibilities of hyperspectral CL imaging for spatially extended specimen such as zircons and other minerals.

White papers

Cathodoluminescence Imaging on Sedimentary Rocks: Quartz Sandstones

Learn how cathodoluminescence spectroscopy can be used for micro-characterization of rocks and minerals, and how this technique can be employed to study quartz sandstone.

White papers

High-resolution Cathodoluminescence Spectroscopy on Shocked Quartz Grains

Read about the possibilities of cathodoluminescence imaging for studying meteorite impacts in shocked quartz grains.

White papers

Complementary Cathodoluminescence and Mass Spectrometry of Australian Sapphires

Read about the research on studying alluvial sapphires from Australia to learn how cathodoluminescence can help to assess crystallisation histories of the precious gemstones and underlying causes for luminescence.

Brochures

SPARC Spectral Brochure

Check exact specifications of the SPARC Spectral, a high-performance cathodoluminescence detector, in the product brochure.

Specification sheets

SPARC Compact Specifications Sheet

Check exact specifications of the SPARC Compact, the cathodoluminescence detector, in the product specifications sheet.

Brochures

SECOM Brochure

Check exact specifications of integrated correlative light and electron microscope SECOM in the product brochure.

Technical notes

Monte Carlo Beam Tracing Simulations for Incoherent CL Emission

Use the databased of Monte Carlo beam tracing simulations for incoherent CL emission for most commonly studied materials to learn how you can set up optimal imaging conditions for your experiments. 

Technical notes

Cathodoluminescence g⁽²⁾ imaging

Learn about time-resolved cathodoluminescence imaging, which can be extremely valuable for a large range of applications.

Technical notes

Energy-Momentum Cathodoluminescence Imaging

Learn the possibilities of lens-scanning energy-momentum imaging mode available in Delmic CL solutions.

White papers

Sample Preparation for Correlative Light and Electron Microscopy

In this white paper, we discuss several methods to ensure an integrated imaging workflow, and present results obtained using a variety of techniques and samples.

Specification sheets

SPARC Spectral Specifications Sheet

Get all specifications of the SPARC Spectral cathodoluminescence detector to get full information about the system.

Specification sheets

JOLT Specifications Sheet

Get all specifications of the panchromatic and RGB cathodoluminescence detector to get full information about the system.

Specification sheets

LAB Cube: lifetime and g(2) imaging module for SPARC spectral

Learn more about SPARC Spectral module for time-resolved cathodoluminescence: lifetime and g(2) imaging.

Publications

CLEM publications

Webinars

2020 Webinar series: Cathodoluminescence fundamentals

 This CL webinar series will go back to the basics of cathodoluminescence imaging and cover its fundamentals as well as the new research directions in six webinar sessions. 

Publications

CL publications

Customer stories

Dating zircons with cathodoluminescence

Meet our customer, Prof. Cameron Davidson, who is currently working at the Department of Geology, Carleton College.

Customer stories

Studying plasmonic antennas with cathodoluminescence

Meet our customer, Dr. Ruggero Verre, who is a post-doctoral researcher in the Department of Applied Physics - Division of Bionanophotonics at the Chalmers University of Technology in Gothenburg in southern Sweden.

Customer stories

Studying plasmonic antennas with cathodoluminescence

Meet our customer, Dr. Sten Littman, who is a scientist working at the department of biogeochemistry in MPI for Marine Microbiology in Bremen.

Customer stories

Looking at impurities of materials and defects with cathodoluminescence

Meet our customer, Dr. Lindgren, who has worked previously as a senior engineer at Sol Voltaics, a start-up which produced solar cells out of nanowires with a new technique called Aerotaxy.

Webinars

Improving cryo-electron tomography workflow with integrated fluorescence light microscopy

Our application specialist Marit Smeets gives an overview of all the steps that are involved in the current sample preparation method, specifically focusing on the role of cryo fluorescence light microscopy (cryo-FLM). We also discuss integrated cryo-FLM and how it can make the workflow easier and more efficient.

Specification sheets

METEOR Specifications Sheet

Learn more about our new integrated cryo-FLM system for FIB/SEM which helps you to make the cryo-CLEM workflow less complex. 

Webinars

Fast EM for large-scale projects

Together with our applications specialist Job Fermie you will dive deep into current challenges of electron microscopy and what would be possible without major limitations.

Webinars

Cathodoluminescence fundamentals: Sample preparation for cathodoluminescence imaging

Our Applications Specialist Sangeetha Hari will introduce the importance of sample preparation for acquiring high quality CL data and describe different sample preparation techniques.

Webinars

High throughput imaging with Delmic’s new Fast EM system

With this webinar, we want to give you a glimpse of what would be possible for your research with Delmic new FAST-EM system and what problems it will help to solve. 

Webinars

Delmic Cryo-ET Journal Club - Episode 1

In the first episode of cryo-ET journal club, our application specialists, Marit Smeets and Caspar Jonker, primarily focus on how the authors used the combination of techniques and how they interpreted the data to come to their conclusions. The discussion touches upon the impact of the paper on cell biology and on cryo-ET technology development.

Webinars

Cathodoluminescence fundamentals: cathodoluminescence imaging for geological materials

This webinar dives into how CL enables fast and high resolution imaging of large areas. The discussion primarily focuses on imaging and analysis techniques for the characterization of zircons and sedimentary rocks. 

Webinars

Delmic Cryo-ET Journal Club Series - The Application of In-situ Cryo-ET to Coronavirus Research

In this episode, our application specialists Marit Smeets and Caspar Jonker discuss two new preprints on bioRxiv, in which the structure and replication of coronaviruses are characterized using in-situ cryo-ET in combination with FIB milling.

Media library

Using cryo fluorescence light microscopy in cryo-ET workflow

Get to know the mechanism of cryo fluorescence light microscopy (cryo-FLM) working in cryogenic electron tomography (cryo-ET). Our application specialist Dr. Caspar Jonker introduced it all in two minutes and explained the possibility to improve the cryo-ET workflow with integrated cryo-FLM.

Customer stories

Integrated CLEM delivers high resolution correlation of fluorescently-tagged molecules to cell organelles

Dr. Lucy M. Collinson and Dr. Christopher J. Peddie are researchers of the Electron Microscopy Science Technology Platform (EM STP) at The Francis Crick Institute in London, specializing particularly in correlative and volume imaging of cells and tissues.

Webinars

Delmic multibeam FAST-EM: technical aspects of the system

In this webinar our applications specialist Job Fermie shows the system's set up, explains how the samples are handled and how the multibeam is set up, generated and detected. We also give a preview of how the image is formed and what data can be acquired with the system.

Application notes

Cathodoluminescence for Plasmonic Nanoantennas

Read this application note to learn how cathodoluminescence can be used for characterization of plasmonic nanoantenna properties.

Application notes

Development of novel UV LED technology using cathodoluminescence inspection

Developing and producing efficient UV LED devices still poses a challenge. Read this application note to learn how cathodoluminescence can be used to inspect UVC LEDs. 

Webinars

Cathodoluminescence fundamentals: Cathodoluminescence imaging for semiconductors

In this webinar, our applications specialist Sangeetha Hari will present exciting results from CL imaging in the field of semiconductors, and explain the hardware and imaging parameters involved.

Technical notes

METEOR technical note: Optical path of the METEOR system

Check out the optical path design of our new integrated cryo-FLM system for FIB/SEM which simplify your cryo-CLEM workflow and increase your lamella yield dramatically.

Webinars

Cathodoluminescence Fundamentals: Correlative cathodoluminescence electron microscopy

In this webinar our applications specialist Sangeetha Hari will talk about cathodoluminescence probes and exciting applications of CCLEM.

Specification sheets

FAST-EM specification sheet

Learn all the technical aspects of FAST-EM in this specification sheet, including electron optics,  scanning and detection, sample and stage, imaging workflow, software, vacuum and support hardware, as well as optional components.

Webinars

Delmic Cryo-ET Journal Club Series (E3): Using cryo-et to understand parkinsons disease

In this journal club we discussed the article The In Situ Structure of Parkinson’s Disease-Linked LRRK2 that tackled this barrier using correlative light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis.

Customer stories

Advancing Scanning Electron Microscopy with FAST-EM

Dr. Jacob P. Hoogenboom is heading one of the ImPhys research groups at the Faculty of Applied Sciences at the TU Delft. The focus of his research group lies in the development of and research with new tools and techniques bridging the gap between light and electron microscopy.

Customer stories

Studying optoelectronic properties of semiconductor thin-films at the nanoscale

Priv.-Doz. Dr. Daniel Abou-Ras, Dr. Klaus Schwarzburg and Dr. rer. nat. Sebastian Schmitt work at Helmholtz-Zentrum Berlin (HZB), a research centre that focuses on studying materials for a sustainable energy supply. Learn how they are using cathodoluminescence to study optoelectronic properties of semiconductor thin films at the nanoscale.

Application notes

Guided cryo-lamellae preparation from yeast by using the integrated fluorescent light microscope METEOR

Check out this application note to learn about the potential of METEOR for simplifying the cryo-ET workflow by preparing targeted cryo-lamellae from Saccharomyces cerevisiae (yeast) overexpressing Ede1 tagged with enhanced green fluorescent protein (eGFP).

Webinars

Delmic Cryo-ET Journal Club Series (E4) - Nature protocol: Cryo-ET sample preparation from whole cells

Our hosts discussed the workflow described by Wagner et al. and compared it to other workflows that have been described over time. Two of the authors, Reika Watanabe and Digvijay Singh, also joined the Q&A session and answered some interesting questions.

Webinars

Tackling ice contamination in cryo-ET

In this webinar, our application specialist Caspar Jonker introduced the crucial steps in the workflow that contribute most to sample ice contamination, and most importantly, the new developments that aim to minimize contamination in cryo-ET altogether.

Customer stories

Understanding fundamental cellular processes with cryo-EM

Dr. Sebastian Tacke and his colleagues working at Max Planck Institute of Molecular Physiology (MPI) innovated the Cryo Workflow Tools, commercially known as CERES Ice Defence System. With the powerful tools, the lab streamlined the cryo-FIB/SEM automation process and reduced the frost contamination by 50% .

Brochures

CERES Ice Defence System Brochure

Download this brochure to learn more about how CERES Ice Defence System, the series of powerful tools, which minimize ice contamination and help you obtain the highest quality cryo-EM samples, with higher throughput and world-class results.

Webinars

Delmic Cryo-ET Journal Club Series (E5) : Using cryo-ET to reveal the molecular architecture of neuronal α-Synuclein inclusions

In this Journal Club, our hosts Dr. Caspar Jonker and Marit Smeets will discuss α-Syn aggregates, which consist of cellular organelles and α-Syn fibrils as shown in the article "In situ architecture of neuronal α-Synuclein inclusions" .

Webinars

Cathodoluminescence imaging of micro and nanoplastics for environmental studies

During this webinar, we will show how CL spectroscopy of sub-micron sized particles can be used on a variety of plastics like polyethylene, PVC, polypropylene, teflon, and mixtures of these materials.

Customer stories

An integrated fluorescent light microscope: a simple and effective solution for streamlining cryo-electron tomography

Anna Bieber, Cristina Capitanio and Oda Helene Schioetz are PhD candidates at the Max Planck Institute of Biochemistry in Martinsried, Germany. By implementing METEOR, they improved the existing workflow to make correlative cryo-focused ion beam milling and cryo-ET more robust, more effective and easier to use.

Customer stories

Investigating plasmonic and dielectric nanostructures with cathodoluminescence

Read the story of our customer, Dr. Saskia Fiedler. She is a Postdoc at SDU (University of Southern Denmark) Nano Optics and The Mads Clausen Institute. Her research is focused on electron beam-based spectroscopy, namely CL and EELS, of plasmonic and dielectric nanostructures.

Application notes

Application note: Cathodoluminescence imaging for characterising micro and nanoplastics

Read the application note to learn how to quantitatively characterize a variety of micro and nanoplastic materials with CL

Media library

Get to know cryogenic electron tomography, a powerful technique revealing biological structures

Get to know Cryogenic electron tomography (cryo-ET), the very powerful technique in two minutes! This technique is becoming more and more important since knowing the location as well as the structure of proteins is crucial for understanding their cellular function.

Brochures

CERES Clean Station Workflow

Download this material to learn more about the CERES Clean Station workflow. Get the technical specifications of the Clean Station and learn how it protects your sample at each step of the workflow.

Webinars

Next generation electron microscopy with FAST-EM

Would you like to make statistically significant conclusions from your EM data, whether that is in pathology, connectomics, toxicology, or cell biology? In this webinar, the Business Unit Owner of Delmic Fast Imaging, Guido Ridolfi, will talk about what could be possible with next-generation electron microscope, FAST-EM.

Webinars

Minimizing sample contamination in cryo-ET with Delmic's CERES Ice Defence System

Would you like to increase your success rate to safely prepare a clean, usable cryo sample? In this webinar our Application Specialist Marit Smeets will discuss the crucial steps in the cryo-ET workflow that contribute most to sample contamination and dive into the new developments that allow reduction of contamination in Cryo-ET altogether. She will present our all-in-one solution: the CERES Ice Defence System.

Brochures

FAST-EM Brochure

Download this brochure to learn more about FAST-EM, an ultra-fast automated multibeam electron microscope.

Webinars

Cathodoluminescence imaging for LEDs and single-photon devices

In this webinar, our Application Specialist Sangeetha Hari focuses on CL imaging for the characterization of LEDs and single-photon devices. She gives practical examples of the two applications that acquired quantitatively comparable data and characterized LEDs from the deep UV to the infrared.

Brochures

METEOR Brochure

Download the brochure to learn more about METEOR, the award-winning fluorescence light microscope that allows monitoring the presence of the target signal during and after milling within the FIB chamber.

White papers

Sample preparation for FAST-EM

Sample preparation is an important part of the electron microscopy workflow. Read the white paper where we discuss the current challenges of sample preparation for electron microscopy and demonstrate how easy it is to use existing automated techniques for sample preparation in combination with FAST-EM.

Application notes

Cathodoluminescence on unicellular marine organisms

Download the application note and learn about the possibilities of cathodoluminescence imaging on unicellular marine organisms.

Customer stories

Accelerating the understanding of Type 1 Diabetes with FAST-EM

Learn how Dr. Ben Giepmans, a principal investigator of University Medical Center Groningen, uses FAST-EM for his research dedicated to Type 1 diabetes.

Specification sheets

CERES Ice Shield Specifications Sheet

Learn more about how to prevent in-chamber ice contamination with CERES Ice Shield.

Technical notes

CERES Ice Shield Ice Contamination Test Protocol

Download the technical note to learn more about the testing protocol of how the sample ice contamination rates will be measured pre and post-CERES Ice Shield Installation.

Customer stories

METEOR and CERES Ice Shield to help shed light on the molecular machinery in organelle membrane and cytoskeletal protein complexes

Meet Delmic's customer, Prof. Dr. Friedrich Förster, who is the head of the in situ Structural Biology Lab, part of the Structural Biochemistry group at Utrecht University, Netherlands.