Studying biological processes at extremely small length scales is becoming increasingly more important in the life sciences. An in-depth understanding of the processes that occur within a cell is, for instance, impossible without intimate knowledge of the various proteins that can be found in a cell. The most effective way to explore this is with the combination of the identification of specific biomolecules with information regarding their precise location in a cell.
Correlative light and electron microscopy (CLEM) is the combination of fluorescence microscopy (FM) and high resolution electron microscopy (EM). The combination of the labelling power of fluorescence imaging and the high resolution structural information provided by electron microscopy makes correlative microscopy the perfect tool to study the complex relation between form and function in biology. Combining the highest resolution fluorescence microscopy within its cellular context is the key to understanding the intricate details of life.
CLEM has a wide variety of applications in the life sciences. This technique allows one to measure both thin sections and cultured cells. See below for more information about specific disciplines for which CLEM can be used.