We have not seen any sign of devitrification in the tomograms of the samples imaged using METEOR. It is known that high power laser sources that are for example used in super resolution applications can devitrify samples1. This is the reason we decided on a LED light source that provides more subtle sample illumination that is not expected to devitrify the sample even at long exposure times. According to our experiments the LED light source is brilliant enough to image biological samples expressing eGFP.
1. Tuijtel, M. W., Koster, A. J., Jakobs, S., Faas, F. G. A. & Sharp, T. H. Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins. Scientific Reports 9, 1–11 (2019).