Correlative light and electron microscopy (CLEM) has a broad range of applications in the life sciences. However, CLEM is particularly useful when objects of interests are sparsely distributed across the sample. The labelling power of the optical microscope combined with the high-resolution structural information provided by the electron microscope provides the ideal means to identify the complex processes
Figure 1: Localization of the lipid diacylglycerol within cellular membranes of HeLa cell expressing GFP-C1.
Figure 3: Human umbilical vein endothelial cells (HUVEC) contain rod-like storage granules called Wiebel-Palade bodies which store Von Willebrand factor (VWF) These organelles play an important role in blood coagulation. Actin (Phalloidin Alexa 488) and VWF (Alexa 568).
Correlative light and electron microscopy is also the perfect method to accurately inspect the morphology and surface topology of cultured cells. With integrated CLEM one has the unique opportunity to simultaneously image fluorescent markers together with all the different types of contrast available for scanning electron microscopy (SEM). This provides a fast and straightforward method to study the cell morphology in correlation with specific proteins of interest.
Growing cells on a substrate