Secom
Request a quoteRequest a demo
SECOM Super-Resolution

Introducing integrated super-resolution correlative light and electron microscopy

The SECOM SR is an integrated platform for correlative light and electron microscopy which uses super-resolution (SR) optical microscopy in combination with an electron microscope. This system possesses all the features of the SECOM system, but is equipped with an extremely powerful optical microscope for yet more detail down to the nanoscale to study the complex relation between form and function in biology.

In recent years, super-resolution microscopy has proven itself to be an extremely powerful technique in biological research. With the invention of SR microscopy, it is now possible to precisely localize biomolecules at length scales that were previously only accessible using electron microscopy. Especially at this length scale, it is important to visualize and understand the micro-environment of these biomolecules; resolution only becomes truly valuable with contextual information.

SECOM Super-Resolution
Localization of the lipid diacylglycerol within cellular membranes of HeLa cell expressing GFP-C1. Sample courtesy C.J. Peddie Francis Crick Institute

Localization of the lipid diacylglycerol within cellular membranes of HeLa cell expressing GFP-C1.

Beat the diffraction barrier

Super-resolution is able to overcome the diffraction barrier of light, which was previously impossible with optical microscopy. The diffraction barrier refers to a law which dictates that it is impossible to identify two different objects that are approximately less than half the wavelength of the light used apart from one another. Ultimately, this means that objects smaller than 200nm cannot be visualized with typical fluorescence microscopy approaches.

Super-resolution microscopy, however, allows one to observe small organelles and proteins. In a super-resolution CLEM system, the electron microscope offers yet more detailed and contextual information to further strengthen the integrity of your research.

Optimize your workflow

Unrivalled overlay accuracy

With the automated overlay procedure of the SECOM SR platform, EM and FM images are always aligned perfectly. Using the automated overlay, time can be spent acquiring rather than aligning data and since the overlay procedure is unbiased it truly strengthens your scientific discoveries. 

Leading optical performance

Because only the highest quality optical components are used, the SECOM SR ensures the best optical performance of any integrated system. One of the unique features is the possibility to use vacuum compatible immersion oil.

High end optics of SECOM SR
Integrated software for CLEM

Modular and easy to use

The SECOM SR is fully compatible with all the standard detectors, allowing you to use the full potential of your electron microscope. Furthermore, special features such as beam deceleration and immersion mode are also supported. Like all DELMIC products, the SECOM SR is compatible with all major SEM manufacturers.

The modular approach in combination with the open-source controlling software ODEMIS ensures a user-friendly solution that can serve a broad user base as well as delivering a system that is a truly unique and an ultimately versatile research instrument.

Dr. Lucy M. Collinson of the Francis Crick Institute

"At our EM Science Technology Platform, [...] we use the SECOM SR platform to perform high accuracy CLEM experiments on resin-embedded cells and tissues containing fluorescent proteins. Implementing superresolution localisation microscopy in the SECOM SR using the blinking of standard fluorescent proteins in-resin takes us to the next level..."

Dr. Lucy M. Collinson, Francis Crick Institute

Download the brochure to read the full testimonial.


Technical notes

Automated overlay technical note for correlative light and electron microscopy
Automated overlay

Applications

Application notes


White paper

Sample Preparation for Correlative Light and Electron Microscopy

One of the challenges associated with integrated CLEM is the preparation of samples suitable for both FM and EM. However, with the right methods one can easily prepare samples in such a way as to be able to benefit fully from correlative microscopy. If you would like to know more about the process of sample preparation for CLEM, you can download our white paper here.

 


Brochure

SECOM SR brochure

If you are interested in the brochure with the exact specifications, you can download it here.


For questions about the SECOM SR, feel free to contact Noor van der Veeken at veeken@delmic.com.